Prevalence of Carbapenemase-Producing Enterobacteriaceae in a University Hospital in Rabat, Morocco: A 19-Months Prospective Study.

Authors

  • Mohammed Adnane EL WARTITI Cheikh Zaid International University Hospital - Faculty of Medicine and Pharmacy, Mohammed V University, Souissi. Rabat, Morocco.
  • Fatima-Zohra BAHMANI Cheikh Zaid International University Hospital - Faculty of Medicine and Pharmacy, Mohammed V University, Souissi. Rabat, Morocco.
  • Mostafa ELOUENNASS Mohammed V Military Teaching Hospital - Faculty of Medicine and Pharmacy, Mohammed V University, Souissi, Rabat, Morocco.
  • Amina BENOUDA Cheikh Zaid International University Hospital - Faculty of Medicine and Pharmacy, Mohammed V University, Souissi. Rabat, Morocco

Keywords:

Carbapenemases, Enterobacteriaceae, Klebsiella, Prevalence, Resistance.

Abstract

Objectives: This study aims to assess the prevalence of clinical isolates of enterobacteriaceae with carbapenem resistance as a result of carbapenemase production, and to characterize the types of enzymes produced.

Methods: All non-duplicate enterobacteriaceae (Klebsiella spp, Enterobacter spp and E. coli) clinical isolates collected over 19 months (i.e., in the period from May 2009 through December 2010) were included in the study. The modified Hodge test was performed on strains showing reduced ertapenem susceptibility. Isolates that tested positive were sent to the French reference center on emerging resistance for molecular characterization and clone determination.

Results: A total of 463 strains were investigated: E. coli 63.9%, Klebsiella spp 27.9% and Enterobacter spp 8.2%. Carbapenemase production occurred in 13 isolates (2.8%). Ten strains produced class D carbapenemases (OXA-48). These were 6 K. pneumoniae, 1 K. oxytoca and 3 E. cloacae. New Delhi metallo-β-lactamase-1 (NDM-1) metallo enzymes were produced by three K. pneumoniae strains.

Conclusion: A source of concern for Morocco can be found in both the high prevalence of carbapenemase producing Klebsiella and Enterobacter clinical isolates and detection of NDM-1 carbapenemases. This requires setting up an emerging resistance monitoring and surveillance scheme at national level.

Author Biographies

Mohammed Adnane EL WARTITI, Cheikh Zaid International University Hospital - Faculty of Medicine and Pharmacy, Mohammed V University, Souissi. Rabat, Morocco.

Microbiology Laboratory (Pharm.D.)

Fatima-Zohra BAHMANI, Cheikh Zaid International University Hospital - Faculty of Medicine and Pharmacy, Mohammed V University, Souissi. Rabat, Morocco.

Microbiology Laboratory (Pharm.D.)

Mostafa ELOUENNASS, Mohammed V Military Teaching Hospital - Faculty of Medicine and Pharmacy, Mohammed V University, Souissi, Rabat, Morocco.

Microbiology Laboratory (DEPARTMENT HEAD, M.D.)

Amina BENOUDA, Cheikh Zaid International University Hospital - Faculty of Medicine and Pharmacy, Mohammed V University, Souissi. Rabat, Morocco

Microbiology Laboratory (DEPARTMENT HEAD, M.D.)

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Published

2012-11-15

Issue

Vol. 2 No. 3 (2012)

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Articles

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