Preparation of Phytol-Loaded Nanoemulsion and Screening for Antioxidant Capacity

  • Md. Torequl Islam PhD student at UFPI, Teresina, Brazil Lecturer at SUB, Chittagong, Bangladesh
  • Leticia Streck
  • Márcia Fernanda Correia Jardim Paz
  • João Marcelo de Castro e Sousa
  • Marcus Vinícius Oliveira Barros de Alencar
  • Ana Maria Oliveira Ferreira da Mata
  • Ricardo Melo de Carvalho
  • Jose Victor de Oliveira Santos
  • Arnobio Antonio da Silva-Junior
  • Paulo Michel Pinheiro Ferreira
  • Ana Amélia de Carvalho Melo-Cavalcante

Abstract

The study aims at preparation of nanoemulsion and subsequently test for antioxidant capacity of a diterpenoid isoprenoid alcohol called phytol (PYT), which is already evident to have a number of important activities. For this PYT-loaded nanoemulsion was prepared by phase inversion method and both PYT-containing nanoemulsion (PNE) and PYT-free nanoemulsion (PFNE) at a concentration range 2 to 16 µM were tested for antiradical (DPPH:  1,1-dipheny-picrylhydrazyl radical; ABTS●+: azino-bis-ethylbenzthiazoline-sulfonic acid; OH: hydroxyl radical scavenging; NO: nitrite oxide radical), lipid peroxidation (LP), reduction potential (RP), and inhibition of hemolysis (HL) in rat erythrocytes activities in comparison of an α-tocopherol analogue, trolox (TRO) (positive control). In addition, an in vivo test was performed in wildtype and deficient Saccharomyces cerevisiae strains using hydrogen peroxide (H2O2) as a stressor (STR). Results suggest that PNE exhibited potential antioxidant activities than the PFNE.  Increased doses of treatment groups showed increased antioxidant capacity in a dose-dependent manner. In the S. cerevisiae study, both PFNE- and PNE-treated groups exhibited decreased rates of survival with the highest doses, whichever in the presence of STR increased the survival rates of strains; suggesting an antioxidative defense capacity of PYT. In this occasion, PNE exhibited prominent antioxidative defense in the presence of STR rather than PFNE. In conclusion, PYT exhibited potential antioxidant activity but at high concentration it is toxic to the yeast cells. The production of PYT-nanoemulsions may be relevant to pharmaceutical sciences.

Published
May 25, 2016
How to Cite
ISLAM, Md. Torequl et al. Preparation of Phytol-Loaded Nanoemulsion and Screening for Antioxidant Capacity. International Archives of Medicine, [S.l.], v. 9, may 2016. ISSN 1755-7682. Available at: <http://imed.pub/ojs/index.php/iam/article/view/1580>. Date accessed: 19 nov. 2017. doi: http://dx.doi.org/10.3823/1941.
Section
Laboratory Medicine

Keywords

antioxidant; radicals; nanoemulsion; phytol; Saccharomyces cerevisiae.

Most read articles by the same author(s)