Micro Rna-499C Induces the Differentiation of Mouse Embryonic Stem Cells (mESCs) into Cardiomyocytes
Background: A microRNA, miR499c, has been discovered in human fetal heart which rescues mutant hearts in cardiac nonfunction mutant axolotl (salamander) embryos and also induces the differentiation of mouse embryonic stem cells (mESCs) to form into definitive cardiomyocytes. Results: Eight days after transfection with MiR499c, approximately 75-80% of the stem cells develop typical cardiomyocyte morphologies and express the cardiac specific marker, Troponin T, as well as the muscle proteins, tropomyosin and α-actinin, as shown by immunohistochemical staining. qRT-PCR confirms that transfection with MiR499c increases expression of troponin T and tropomyosin and further shows an increased expression of myosin as well as Wnt11 and Sox17. Untreated controls do not show significant expression of these proteins. Conclusion: It is evident that the miR499c induces the development of contractile myofibrils characteristic of striated cardiac muscle indicating that the miR499c microRNA plays an important role in the differentiation of cardiomyocytes.
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access and Benefits of Publishing Open Access).