Antimicrobial Resistance Patterns of Diarrheagenic and non-Diarrheagenic Escherichia coli Isolates from Libyan Children
Keywords:
Diarrhoeagenic E.coli, antimicrobial-resistance genes, LibyaAbstract
Background: Diarrhea continues to be one of the most common causes of morbidity and mortality among infants and children in developing countries. This study was carried out among Libyan children in Benghazi to investigate the prevalence of diarrheagenic E.coli types and antimicrobial susceptibility profiles of E.coli isolates from their stools.
Methods  & patients: A total of 200 children with diarrhea and 90 without diarrhea were investigated for presence of E.coli in their stools using culture on MacConkey agar, and the isolates were examined for detection diarrheagenic E. coli types, antimicrobial susceptibility pattern, presence of ESBL-CTX-M genes and integrons using PCR .
Results: The study indicated that diarrhoeagenic E. coli isolates were  found only in stools of children with diarrhoea. The detected diarrhoeagenic  E. coli types; were 4 (2%) enteroaggregative E. coli (EAEC), 3 (1.5%) enteroinvasive E. coli (EIEC), and 1(0.5%)  enterohemorrhagic E. coli (EHEC), and both enterotoxigenic E. coli(ETEC) and enteropathogenic E. coli(EPEC) were not present. It has also found that multidrug resistant E.coli (> 3 drugs) was higher prevalent in commensal intestinal E.coli than in diarrheagenic E.coli, and both carried high rates of Class 1 integrons, bla(CTX-M) and fluroquinolone resistance genes .
Conclusion: This study revealed the low incidence of diarrhoeagenic E. coli isolates and high prevalent of antimicrobial resistance among normal intestinal E.coli of hospitalized children in Benghazi/Libya.
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