Co-existance of Aminoglycoside Modifying Enzymes (AMEs) genes and mec-A gene among nosocomial isolates of Staphylococcus aureus in Surgical Intensive Care Units in Kasr Al-Ainy hospitals, Cairo University.

  • Ashraf E. Sorour Department of Medical Microbiology & Immunology, Faculty of Medicine, Cairo University; Cairo, Egypt.
  • Basma A El-Awady Department of Medical Microbiology & Immunology, Faculty of Medicine, Cairo University; Cairo, Egypt
  • Mukhtar AM Department of Anesthesia & Intensive Care; Faculty of Medicine, Cairo University; Cairo, Egypt

Abstract

In Staphylococcus aureus aminoglycosides and methicillin resistances are closely associated. We aimed at detection of the prevalence of methicillin and aminoglycosides resistances in S. aureus isolated from  surgical ICUs in Kasr Alainy hospitals,  the association of mecA gene and aminoglycoside modifying enzymes (AMEs)  genes among these isolates and the accuracy of cefoxitin disc diffusion method in relation to PCR.

150 clinical samples were collected. Cultivation and  identification of isolates were done by the standard   microbiological techniques. All S. aureus isolates were tested for methicillin and aminoglycosides  resistance by disc diffusion  method and for presence  of mec A gene  and AMEs genes by multiplex-PCR.

48 S. aureus were isolated (32%) with  a high prevalence of mec A gene  (89.58%) and AME  genes (60.42%) and co-existance of mec A gene and one or more of  AME genes in 60%.   mec A gene was detected  in 87% of cefoxitin  resistant  isolates  and  aac(6')-Ie+aph(2'') was the most predominant AME genes. 

Published
Jan 17, 2014
How to Cite
SOROUR, Ashraf E.; EL-AWADY, Basma A; AM, Mukhtar. Co-existance of Aminoglycoside Modifying Enzymes (AMEs) genes and mec-A gene among nosocomial isolates of Staphylococcus aureus in Surgical Intensive Care Units in Kasr Al-Ainy hospitals, Cairo University.. The International Arabic Journal of Antimicrobial Agents, [S.l.], v. 3, n. 4, jan. 2014. ISSN 2174-9094. Available at: <http://imed.pub/ojs/index.php/IAJAA/article/view/772>. Date accessed: 25 jan. 2021.
Section
Articles

Keywords

S. aureus - mec A gene - aminoglycoside modifying enzymes genes -PCR