Antimicrobial tolerance changes in biocide-passaged biofilm cultures of Pseudomonas aeruginosa PAO1
AbstractThe aim of this study was to develop a novel process for the passaging of biofilms of Pseudomonas aeruginosa towards tolerance to a group of selected antimicrobial agents (biocides). The combination of both a traditional passage approach coupled with a suitable biofilm model, for the production of large numbers of adherent cells, yielded evidence of a development of phenotypic tolerance in Ps. aeruginosa towards selected biocides (zinc pyrithione (ZnPT), sodium pyrithione (NaPT), Cetrimide & Benzisothiazolone (BIT)). The results indicate a step-wise increase in minimal inhibitory concentration (MIC) over a series of ten consecutive passages in the presence of increasing concentrations of biocide. Scanning electron microscopy revealed a complex internal structure to the biofilms grown using this model. Results indicate that it is possible to passage cultures of Ps. aeruginosa towards phenotypic tolerance to selected antimicrobial agents. Results also indicate that such tolerance is partially reversible and that some residual tolerance remains in the absence of biocide. Thus, indicating that the tolerance is partially due to phenotypic changes within biofilm cells. This study describes a novel technique for the induction of phenotypic tolerance towards antimicrobial agents in biofilm situations. The model used here may be adapted to other areas of biofilm work, where clonal selection of phenotypic traits may be desirable.
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access and Benefits of Publishing Open Access).
This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge.
Articles are published Under License of Creative Commons Attribution 3.0 License ©
Copyright policies & self-archiving
|Author's Pre-print:||author can archive pre-print (ie pre-refereeing)|
|Author's Post-print:||author can archive post-print (ie final draft post-refereeing)|
|Publisher's Version/PDF:||author can archive publisher's version/PDF|