Identification of the first bacteriocin isolated in Lebanon extracted via a modified adsorption-desorption method and its potential food application

Authors

  • Imad Al Kassaa Laboratoire de Microbiologie Santé et Environnement (LMSE), Doctoral School of Sciences and Technology , Faculty of Public Health, Lebanese University, Tripoli, Lebanon
  • Mazen ZAYLAA Laboratoire de Microbiologie Santé et Environnement (LMSE), Doctoral School of Sciences and Technology , Faculty of Public Health, Lebanese University, Tripoli, Lebanon
  • Samah Hussein Laboratoire de Microbiologie Santé et Environnement (LMSE), Doctoral School of Sciences and Technology , Faculty of Public Health, Lebanese University, Tripoli, Lebanon
  • Khaled El Omari Quality Control Center Laboratories at the Chamber of Commerce, Industry & Agriculture of Tripoli & North Lebanon
  • Nour-Eddin Chihib UMR CNRS 8207, Unité des Matériaux Et Transformations (UMET), Equipe Processus aux Interfaces et Hygiène des Matériaux (PIHM), CNRS-INRA, Institut Chevreul, Université de Lille, France
  • Monzer Hamze Laboratoire de Microbiologie Santé et Environnement (LMSE), Doctoral School of Sciences and Technology , Faculty of Public Health, Lebanese University, Tripoli, Lebanon
  • Hoda Yusef , Faculty of Science,Department of Biological and Environmental Sciences Beirut Arab University, Beirut, Lebanon
  • Fouad Dabboussi Laboratoire de Microbiologie Santé et Environnement (LMSE), Doctoral School of Sciences and Technology , Faculty of Public Health, Lebanese University, Tripoli, Lebanon

DOI:

https://doi.org/10.3823/817

Keywords:

Dairy product, Enterocin, Food preservation, Lactic acid bacteria, Listeria

Abstract

Introduction: The raw goat milk is considered as a good source of novel bacteriocinogenic lactic acid bacteria (LAB) strains.

Method: The bacteriocin, which named enterocin CMUL20-2 was secreted by Enterococcus faecium CMUL20-2. This bacterial strain was originally isolated from raw goat’s milk, was extracted by using a modified adsorption-desorption method and purified via RP-HPLC. antimicrobial activity was tested against several pathogenic and spoilage microbes.

Results: The enterocin CMUL20-2 showed a strong adsorption on cell wall of producer strain even in acidic environment which facilitate its extraction in only two simple steps. The recovered purified enterocin has decreased procedure time and diminished the number of undesirable molecules present in Rogosa and Sharpe (MRS) broth. The recovered enterocin showed antimicrobial activity against several foodborne pathogenic and spoilage microbes.

Conclusion:  The recovered enterocin was able to tolerate a variety of food chain conditions such as high temperature, pH and storage stability, and it can be a good candidate to protect food from spoilage microbes

 

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2018-03-19

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