Identification of the first bacteriocin isolated in Lebanon extracted via a modified adsorption-desorption method and its potential food application
Keywords:Dairy product, Enterocin, Food preservation, Lactic acid bacteria, Listeria
Introduction: The raw goat milk is considered as a good source of novel bacteriocinogenic lactic acid bacteria (LAB) strains.
Method: The bacteriocin, which named enterocin CMUL20-2 was secreted by Enterococcus faecium CMUL20-2. This bacterial strain was originally isolated from raw goatâ€™s milk, was extracted by using a modified adsorption-desorption method and purified via RP-HPLC. antimicrobial activity was tested against several pathogenic and spoilage microbes.
Results: The enterocin CMUL20-2 showed a strong adsorption on cell wall of producer strain even in acidic environment which facilitate its extraction in only two simple steps. The recovered purified enterocin has decreased procedure time and diminished the number of undesirable molecules present in Rogosa and Sharpe (MRS) broth. The recovered enterocin showed antimicrobial activity against several foodborne pathogenic and spoilage microbes.
Conclusion: The recovered enterocin was able to tolerate a variety of food chain conditions such as high temperature, pH and storage stability, and it can be a good candidate to protect food from spoilage microbes
Konings WN, Kok J, Kuipers OP, Poolman B: Lactic acid bacteria: the bugs of the new millennium. Curr Opin Microbiol 2000;3:276â€“282.
Masood MI, Qadir MI, Shirazi JH, Khan IU: Beneficial effects of lactic acid bacteria on human beings. Crit Rev Microbiol 2011;37:91â€“98.
Kassaa IA: New Insights on Antiviral Probiotics: From Research to Applications. Springer, 2016.
Jack RW, Tagg JR, Ray B: Bacteriocins of gram-positive bacteria. Microbiol Rev 1995;59:171â€“200.
Noordiana N, Fatimah AB, Mun AS: Antibacterial agents produced by lactic acid bacteria isolated from Threadfin Salmon and Grass Shrimp. Int Food Res J 2013;20:117â€“124.
. Engelbrecht F, DomÃnguez-Bernal G, Hess J, Dickneite C, Greiffenberg L, Lampidis R, et al.: A novel PrfA-regulated chromosomal locus, which is specific for Listeria ivanovii, encodes two small, secreted internalins and contributes to virulence in mice. Mol Microbiol 1998;30:405â€“417.
Bodro M, Paterson DL: Listeriosis in patients receiving biologic therapies. Eur J Clin Microbiol Infect Dis Off Publ Eur Soc Clin Microbiol 2013;32:1225â€“1230.
Franciosi E, Settanni L, Cavazza A, Poznanski E: Biodiversity and technological potential of wild lactic acid bacteria from raw cowsâ€™ milk. Int Dairy J 2009;19:3â€“11.
Ortolani MBT, Moraes PM, Perin LM, ViÃ§osa GN, Carvalho KG, Silva JÃºnior A, et al.: Molecular identification of naturally occurring bacteriocinogenic and bacteriocinogenic-like lactic acid bacteria in raw milk and soft cheese. J Dairy Sci 2010;93:2880â€“2886.
RodrÄ±Ìguez E, GonzÃ¡lez B, Gaya P, NuÃ±ez M, Medina M: Diversity of bacteriocins produced by lactic acid bacteria isolated from raw milk. Int Dairy J 2000;10:7â€“15.
Ogunbanwo ST, A.i S, Onilude AA: Characterization of bacteriocin produced by Lactobacillus plantarum F1 and Lactobacillus brevis OG1. Afr J Biotechnol 2003;2:219â€“227.
Toba T, Samant S k., Itoh T: Assay system for detecting bacteriocin in microdilution wells. Lett Appl Microbiol 1991;13:102â€“104.
Cherif A, Rezgui W, Raddadi N, Daffonchio D, Boudabous A: Characterization and partial purification of entomocin 110, a newly identified bacteriocin from Bacillus thuringiensis subsp. Entomocidus HD110. Microbiol Res 2008;163:684â€“692.
Stiles ME, Holzapfel WH: Lactic acid bacteria of foods and their current taxonomy. Int J Food Microbiol 1997;36:1â€“29.
Al Kassaa I, Hamze M, Hober D, Chihib N-E, Drider D: Identification of vaginal lactobacilli with potential probiotic properties isolated from women in North Lebanon. Microb Ecol 2014;67:722â€“734.
Yang R, Johnson MC, Ray B: Novel method to extract large amounts of bacteriocins from lactic acid bacteria. Appl Environ Microbiol 1992;58:3355â€“3359.
Sharma N, Kapoor G, Neopaney B: Characterization of a new bacteriocin produced from a novel isolated strain of Bacillus lentus NG121. Van Leeuw J Microbiol 2006;89:337â€“343.
Brink B, Minekus M, van der Vossen JM, Leer RJ, Huis inâ€™t Veld JH: Antimicrobial activity of lactobacilli: preliminary characterization and optimization of production of acidocin B, a novel bacteriocin produced by Lactobacillus acidophilus M46. J Appl Bacteriol 1994;77:140â€“148.
Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing, 28th Edition. 950 West Valley Road, Suite 2500 Wayne, PA 19087 USA, 2018.
ChajÄ™cka-Wierzchowska W, Zadernowska A, Åaniewska-Trokenheim Å: Virulence factors, antimicrobial resistance and biofilm formation in Enterococcus spp. isolated from retail shrimps. LWT - Food Sci Technol 2016;69:117â€“122.
Macovei L, Zurek L: Ecology of antibiotic resistance genes: characterization of enterococci from houseflies collected in food settings. Appl Environ Microbiol 2006;72:4028â€“4035.
Yanagida F, Chen Y, Onda T, Shinohara T: Durancin L28-1A, a new bacteriocin from Enterococcus durans L28-1, isolated from soil. Lett Appl Microbiol 2005;40:430â€“435.
Wijaya A: Investigation into the influence of a bacteriocin-producing Enterococcus strain on the intestinal microflora. PhD Dessertation 2003
Ray B, Schamber R, Miller KW: The pediocin AcH precursor is biologically active. Appl Environ Microbiol 1999;65:2281â€“2286.
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access and Benefits of Publishing Open Access).
This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge.
Articles are published Under License of Creative Commons Attribution 3.0 License Â©
Copyright policies & self-archiving
|Author's Pre-print:||author can archive pre-print (ie pre-refereeing)|
|Author's Post-print:||author can archive post-print (ie final draft post-refereeing)|
|Publisher's Version/PDF:||author can archive publisher's version/PDF|